Tag

Part:BBa_K2368015:Design

Designed by: BIT-China 2017   Group: iGEM17_BIT-China   (2017-10-01)


T1R2-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Firstly, we construct the specific primer that consists of the overlap regions of the T1R2 and GCP. Then, we combine T1R2 with the GFP by overlap extension PCR.


Source

References